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Wednesday, May 12, 2010

STANDARD OPERATING PROCEDURE Title: Preparation of Disinfectant

Purpose:
To describe the procedure for the preparation & use of the disinfectants to be used in microbiology laboratory.

2. Scope:
This procedure is applied when the preparation of the disinfectants is required.

3. Responsibilities:

1. Microbiologist
2. Lab Assistant

4. Procedure:

A. DISINFECTANTS

1. PHENOL 5% V/V (Carbolic acid)
Phenol 5.5 ml
Distilled water 100 ml q.s. (Can be sterilized for sterile areas at 121°C for 30 min.)
This concentration is normally used for mopping walls, floors, table tops, gloves and outer surfaces of sample containers and Petri-dishes etc.
Adverse Effects
They are poisonous if swallowed.
Causes chemical burns if directly contacted with skin.
The odour is highly irritating.
They may stain wool, cotton and synthetic fabrics.
Storage
Sterilized Phenol can be stored for one month.

2. ALCOHOL 70% V/V
Isopropyl Alcohol 74 ml
Distilled water 26 ml to make 100 ml of 70% Alcohol. (It is filtered for sterile area use).
They are normally used where prolonged disinfection is not required. This concentration is used to disinfect the gloves, surfaces of Laminar Flows, outer surfaces of samples containers and Petri-dishes etc.
Adverse Effects
Alcohols are toxic and irritant to skins and generally unsuitable for application to mucous membranes.
Rubber swells and plastics may be hardened by frequent or prolonged contact with alcohols.
Storage
Filtered Alcohol can be stored up to one week.

3. DETTOL 2% V/V (Chloroxylenol)

Dettol concentrated 20 ml
Distilled water 980 ml
It can be used as disinfecting surfaces, gloves, outer surfaces of sample containers etc.
Adverse Effects
Same as of Phenols at high concentrations.

4. FORMALIN 5%

Formalin (37-40%) 70 ml
Make up the volume to 500 ml
It is basically used for fumigation purposes in sterile areas in Pharmaceutical industries. The spray after work to disinfect hazardous microbes is recommended.
Adverse Effects
Vapours are extremely irritant to the eyes and respiratory tract at 2-5 p.p.m conc.
Direct contact causes skin irritation and allergic dermatitis.

B. PERSONAL PROTECTION
Take all necessary safety measures during preparation of disinfectants because they all are toxic to human beings too.
Wear goggles.
Wear appropriate protective gloves.
Wear respiratory full face mask with appropriate filter where applicable.

C. EMERGENCY CASES
Immediately inform the supervisor.
In case of injury contact for medical help

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